Year: 2015 | Month: April | Volume 5 | Issue 1
Detection of Virulence Genes in Salmonella Species Isolated from Chevon and Chicken Meat
Abstract:
The aim of present study was to investigate the detection of virulence factors encoded Salmonella enterotoxin (stn) and plasmid encoded fimbriae (pef) genes in isolates of Salmonellae isolated from chevon and chicken meat samples collected from different districts of Chhattisgarh. A total of 32 Salmonella isolates were recovered, among them 18 and 14 isolates were recovered from chevon and chicken meat samples, respectively. All the Salmonella isolates were tested for the presence of virulence associated genes viz., stn and pef by using polymerase chain reaction (PCR) protocols, standardized separately for each gene. All 32 (100%) Salmonella isolates were found to carry the enterotoxin determinant stn gene where as none of the Salmonella isolate was found positive for pef gene, indicating that the stn gene is widely distributed among the Salmonella isolates irrespective of source of sample, species, serovars and location. The stn gene in Salmonella was found to be highly conserved. Thus, stn gene may be used as a target gene for detection of Salmonellae in different types of field samples.
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